rabbit anti synaptotagmin  (Cell Signaling Technology Inc)

Journal: Journal of Biochemical and Molecular Toxicology

Article Title: Bisphenol A Treatment Impairs Synaptic Function in Human Cholinergic Neurons

doi: 10.1002/jbt.70558

Figure Lengend Snippet: Densitometric analysis of PSD95, SYT, and SYP protein levels of CTRL and BPA‐treated cells. The level of ACTIN protein was used as a loading control for protein normalization in western blot analysis. For both CTRL and BPA‐treated samples, each lane represents an independent biological replicate of the experiment. Values are reported as mean ± SEM. * p ≤ 0.05, ** p ≤ 0.01. Uncropped gels are provided in the Figure .

Article Snippet: Following blocking, the membranes were incubated overnight, at 4°C with primary antibodies against SYP (AB9272; Merk‐Millipore; dilution 1:80,000), SYT (#14558; Cell Signaling Technology; dilution 1:1000), PSD‐95 (#2507; Cell Signaling Technology; dilution 1:1000), PARP‐1 (AB‐83632; Immunological Science; dilution 1:1000), and CASP‐3 (sc‐271028; Santa Cruz; dilution 1:1000).

Techniques: Control, Western Blot

Journal: Sexual Medicine

Article Title: Yimusake ameliorates corporal endothelial dysfunction by down-regulating the NLRP3 inflammasome–mediated NF-κB signaling pathway and inhibiting oxidative stress

doi: 10.1093/sexmed/qfaf079

Figure Lengend Snippet: Yimusake can inhibit the expression of the NLRP3 inflammasome-mediated NF-κB signaling pathway in vitro . (A) Western blotting was used to determine the expression of the NLRP3, p65, p-p65, IκBα, p-IκBα, and IL-18 protein expression in CCECs of each group. (B) Relative expression of NLRP3. (C) Relative expression of p-p65/p65. (D) Relative expression of p-IκBα/IκBα. (E) Relative expression of IL-18 (F) PCR detection of NLRP3 mRNA expression in CCECs. (G) RT-qPCR detection of p65 mRNA expression in CCECs, * P < .05 compared with the NC group, # P < .05 compared with the HG group, & P < .05 compared with the Sh-NLRP3 group, $ P < .05 compared with the Y group ( P < .05).

Article Snippet: Primary antibodies including rabbit anti-NF-κB p65 (1:100, BA0610, Boster, China) were added and incubated overnight at 4 °C.

Techniques: Expressing, In Vitro, Western Blot, Quantitative RT-PCR

Journal: Sexual Medicine

Article Title: Yimusake ameliorates corporal endothelial dysfunction by down-regulating the NLRP3 inflammasome–mediated NF-κB signaling pathway and inhibiting oxidative stress

doi: 10.1093/sexmed/qfaf079

Figure Lengend Snippet: Yimusake can inhibit the expression of the NLRP3 inflammasome-mediated NF-κB signaling pathway in vivo. (A) Immunohistochemical detection of NF-κB p65 protein expression in rat penile tissue (x200). (B) Relative expression of p65. (C) Western blotting was used to determine the expression of the p65, p-p65, IκBα, p-IκBα, and IL-18 protein expression in rat penile tissue of each group. (D) Relative expression of p-p65/p65. (E) Relative expression of p-IκBα/IκBα, (F) relative expression of IL-18. (G) RT-qPCR detection of p65 mRNA expression in rat penile tissue, * P < .05 compared with the NC group, # P < .05 compared with the DMED group, & P < .05 compared with the MCC950 group, $ P < .05 compared with the Y group.

Article Snippet: Primary antibodies including rabbit anti-NF-κB p65 (1:100, BA0610, Boster, China) were added and incubated overnight at 4 °C.

Techniques: Expressing, In Vivo, Immunohistochemical staining, Western Blot, Quantitative RT-PCR